發布於 2018-01-11
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  • 00:25 1.
    Genetic Analysis and Mapping in Bacteria and Bacteriophages
  • 01:16 2.
    Genetic Analysis and Mapping in Bacteria and Bacteriophages
  • 04:39 3.
    Mutation
  • 06:23 4.
    Phenotypic Classes of Mutations
  • 13:31 5.
    Mutation arisen from adaption or spontaneity
  • 02:05 6.
    Fluctuation Test
  • 09:50 7.
    Fluctuation Test
  • 00:46 8.
    Assumptions
  • 00:33 9.
    Fluctuation Test Results
  • 01:21 10.
    Why large variation?
  • 02:40 11.
    Selection vs. counter-selection
  • 06:21 12.
    Biochemical or nutritional mutation
  • 08:33 13.
    Slide 12
  • 01:34 14.
    Three processes are involved in the transfer of genetic information between one bacterium to another
  • 01:37 15.
    Bacteria undergo conjugation, in whichgenetic information from one bacterium is transferred to another it recombines with the second bacterium's DNA
  • 09:37 16.
    Conjugation was first demonstrated by Lederberg and Tatum (1946)
  • 04:47 17.
    Bernard Davis’s experiment:
  • 11:45 18.
    Slide 17
  • 02:00 19.
    Hfr bacteria and chromosome mappingCavalli-Sforza (1950)
  • 00:52 20.
    Slide 17
  • 02:17 21.
    Hfr bacteria and chromosome mappingCavalli-Sforza (1950)
  • 10:28 22.
    Slide 19
  • 02:34 23.
    Interrupted matings demonstrated that specific genes in an Hfr strain are transferred and recombined sooner than others
  • 00:05 24.
    Slide 21
  • 00:02 25.
    Interrupted matings demonstrated that specific genes in an Hfr strain are transferred and recombined sooner than others
  • 01:03 26.
    Slide 21
  • 00:11 27.
    Slide 22
  • 00:00 28.
    Slide 21
  • 00:00 29.
    Interrupted matings demonstrated that specific genes in an Hfr strain are transferred and recombined sooner than others
  • 01:53 30.
    Slide 19
  • 00:00 31.
    Interrupted matings demonstrated that specific genes in an Hfr strain are transferred and recombined sooner than others
  • 00:00 32.
    Slide 21
  • 03:13 33.
    Slide 22
  • 05:05 34.
    F’ and merozygote
  • 07:14 35.
    Plasmids
  • 21:51 36.
    Slide 25
  • 19:58 37.
    Genes that are close enough to each other to be cotransformed are linked (genes are close to or next to each other)Two unlinked genes are cotransformed only as a result of two independent events involving two distinct segments of DNA. In such a case, the
  • 02:41 38.
    Demonstration of linked genes in Pneumococcus
  • 03:56 39.
    Bacteriophages Are Bacterial Viruses
  • 06:50 40.
    Slide 29
  • 17:30 41.
    The number of phages produced following the infection of bacteria can be determined by the plaque assayThis technique entails performing serial dilutions of virally infected bacteria, which are then poured onto agar platesBy counting the number of plaques
  • 06:04 42.
    Lysogeny (溶原性)
  • 03:11 43.
    Slide 32
  • 04:30 44.
    Transduction
  • 06:05 45.
    Filterable agent (FA)
  • 01:47 46.
    Salmonella
  • 00:01 47.
    Slide 36
  • 07:11 48.
    Salmonella
  • 02:34 49.
    Slide 36
  • 01:31 50.
    Transduction
  • 12:25 51.
    Specialized Transduction: Phage 
  • 01:40 52.
    Generalized transduction results in transfer of a large number of bacterial genes-92 kbSpecialized transduction results in transfer of only a few bacterial genesLike transformation, generalized transduction can be used in linkage and chromosomal mapping-c
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Genetic-analysis-and-mapping-in-bacteria-and-bacteriophages-2017
4:04:55, 發布於 2018-01-11 by 呂佳樺